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uea 1 rhodamine  (Vector Laboratories)


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    Structured Review

    Vector Laboratories uea 1 rhodamine
    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
    Uea 1 Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/RL-1062-2/Rhodamine+labeled+Ulex+Europaeus+Agglutinin+I+(UEA+I)/bio_rxiv__64898__2026__04__15__718752-82-23-26
    Average 94 stars, based on 194 article reviews
    uea 1 rhodamine - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa"

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa

    Journal: bioRxiv

    doi: 10.64898/2026.04.15.718752

    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
    Figure Legend Snippet: ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Techniques Used: Staining

    Related Articles

    Immunopeptidomics:

    Article Title: Human gut M cells resemble dendritic cells and present gluten antigen.
    Article Snippet: Microfold (M) cells are rare intestinal epithelial cells that reside in the follicle-associated epithelium of Peyer’s patches.. M cells transport luminal antigens to submucosal antigen-presenting cells.. These insights primarily derive from transmission electron microscopy and studies using genetically modified mice.

    Article Title: Human gut M cells resemble dendritic cells and present gluten antigen
    Article Snippet: Leica LAS X software (v.3.5.7.23225) was used for image processing and export. .. Primary antibodies used in this study: mouse anti-human GP2 (MBL, D277-3, clone 3G7-H9, diluted 1:200); mouse anti-human ICAM2 (Thermo Fisher, BMS109BT, clone CBR-IC2/2, diluted 1:200); mouse anti-human HLA-DP/DQ/DR (Thermo Fisher, MA1-25914, clone CR3/43, diluted 1:200); rat anti-mouse MHC-II (I-A/I-E) (Thermo Fisher, 14-5321-82, clone M5/114.15.2, diluted 1:200), UEA-1-Rhodamine (Vector, RL-1062-2, diluted 1:1,000); Phalloidin (Sigma, 65906-10NMOL, diluted 1:1,000); DONQ52 antibody (provided by Chugai Pharmaceutical, diluted 1:100). .. IHC staining images using TFF2 and MHC-II antibodies in primary human intestinal tissues were obtained from the Human Protein Atlas ( https://proteinatlas.org ), details regarding antibodies and patient numbers are included in the related figures.

    Labeling:

    Article Title: RAB3GAP2 is a regulator of skeletal muscle endothelial cell proliferation and associated with capillary-to-fiber ratio.
    Article Snippet: .. For endothelial cell identification, sections for IF were counterstained either with 2.0 μg/ml mouse antiCD31 (Dako, Glostrup, Denmark) and Alexa488 conjugated goat anti-mouse IgG (1:400; Abcam) and DAPI (4’,6-diamidino-2-phenylindole) for nuclear identification (Vector Laboratories, Burlingame, CA, US); or with Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I) (Vector Laboratories) (10 μg/ml). .. Sections were mounted and imaged in a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

    Incubation:

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa
    Article Snippet: For Lectin staining, slides were deparaffinized in xylene, rehydrated through a graded ethanol series, and blocked for endogenous biotin/streptavidin binding using a Streptavidin/Biotin blocking kit (Vector Laboratories). .. Sections were incubated with biotinylated MAL-II (B-1265-1, Vector Laboratories; 3μg/mL in 1%BSA in PBS) overnight at 4°C, washed in PBS, then incubated with UEA-1 rhodamine (RL-1062-2, Vector Laboratories; 2μg/mL) and streptavidin-FITC (405201, BioLegend; 5μg/mL) in 1% BSA in PBS for 1 hr at room temperature in the dark. ..



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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    Image Search Results


    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Journal: bioRxiv

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa

    doi: 10.64898/2026.04.15.718752

    Figure Lengend Snippet: ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Article Snippet: Sections were incubated with biotinylated MAL-II (B-1265-1, Vector Laboratories; 3μg/mL in 1%BSA in PBS) overnight at 4°C, washed in PBS, then incubated with UEA-1 rhodamine (RL-1062-2, Vector Laboratories; 2μg/mL) and streptavidin-FITC (405201, BioLegend; 5μg/mL) in 1% BSA in PBS for 1 hr at room temperature in the dark.

    Techniques: Staining